产品编号:ALR-001-500ug
产品描述:Recombinant Phospholipase A2(Phospholipase A2)
反应种属:
实验方法:
标记:
规格:500µg
供应商:Prospec
价格(RMB):8400.00
订购:
说明书:
Phospholipase A2
Phospholipase A2 P00630 Bee Venom Protein Recombinant
Catalog No. ALR-001
Synonyms:
Phospholipase A2, Phosphatidylcholine 2-acylhydrolase, Allergen Api m I, Api m 1.
Introduction:
Bee venom phospholipase A2 (PLA) is the main allergen in the bee sting allergy. Bee venom phospholipase A2 (BV-PLA2) is a hydrolytic enzyme which specifically cleaves the sn-2 acyl bond of phospholipids at the lipid/water interface. BV-PLA2 is a 14–16-kDa glycoprotein, consisting of 134 amino acids and displaying a single carbohydrate side chain at the residue Asn13. It is also held to be responsible for some systemic anaphylactic reactions in bee venom sensitized individuals. BV-PLA2 presents 3 peptide and a glycopeptide T cell epitopes, which are recognized by both allergic and non-allergic bee venom sensitized subjects. PLA is able to elicit both IgE mediated allergy and normal immunity to bee sting which usually is associated with high affinity IgG4 anti-PLA antibodies.
Description:
The E.Coli derived recombinant protein contains phospholipase P00630 bee venom protein epitopes, 26-162 amino acids.
Method:
Purified by proprietary chromatographic technique.
Purity:
Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.
Formulation:
10mM Tris-HCl, pH 6.5, 0.1M Sodium Phosphate and 8M urea.
Storage:
Protein is shipped at ambient temperature.
Upon arrival, Store at -20°C.
Stability:
Five years frozen. One month in solution at room temperature.
Applications:
Use as an antigen in ELISA and Western Blots.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
