产品编号:PKA-324-1mg

产品描述:Recombinant Human PKC-q(PKC-q)

反应种属:

实验方法:

标记:

规格:1mg

供应商:Prospec

价格(RMB):67200.00

订购:

说明书:

PKC-t Human
Protein Kinase C theta Human Recombinant
Catalog No. PKA-324

Synonyms:
Protein kinase C theta type, EC 2.7.11.13, nPKC-theta, PRKCQ, PRKCT, MGC126514, MGC141919.

Introduction:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.

Description:
PKC-q Human Recombinant produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-706 and having a molecular mass of 86 KD. This protein is the full-length form of the protein with an amino terminal poly His-tag.
PKC-q is purified by proprietary chromatographic techniques.

Source:
Sf9 insect cells.

Physical Appearance:
Sterile Filtered clear solution.

Formulation:
PKC-q is supplied at a of 0.1mg/ml in 10mM Tris, pH 7.4, 0.1M NaCl, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF and 0.03% Brij-35.

Unit Definition:
~ 1800 units/mg. One unit of PKCq activity transfers 1 nmole of phosphate to a PKCe pseudosubstrate peptide per minute at 30°C using. Recombinant human active PKCq also phosphorylates histone, MARCKS, moesin, RasGRP3, and PKCa and PKCe pseudosubstrates. PKCq is a novel PKC and does not require calcium for activity. Activity may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application. PKCq is involved in T-cell activation and T-cell receptor signaling including T-cell mediated activation of NF-kB and AP-1.

Storage Conditions:
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
 
Purity:
Greater than 85% as determined by SDS-PAGE.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH
USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.