产品编号:PKA-207-20ug

产品描述:Recombinant Human PKAkt1/PKBa(PKAkt1/PKBa)

反应种属:

实验方法:

标记:

规格:20µg

供应商:Prospec

价格(RMB):12600.00

订购:

说明书:

PKAkt1/PKBa Human
Protein Kinase Akt1/PKB alpha, Inactive enzyme Human Recombinant
Catalog No. PKA-207

Synonyms:
RAC-alpha serine/threonine-protein kinase, EC 2.7.11.1, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, AKT1, AKT, RAC, PRKBA, MGC99656, RAC-ALPHA.

Introduction:
Akt1, also known as "Akt" or protein kinaseB (PKB) is an important molecule in mammaliancellular signaling.
In humans, there are three genes in the "Akt family": Akt1, Akt2, and Akt3. These enzymesare members of the serine/threonine-specific protein kinasefamily (EC2.7.11.1).
Akt1 is involved in cellular survival pathways, by inhibiting apoptoticprocesses. Akt1 is also able to induce protein synthesispathways, and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth. Since it can block apoptosis, and thereby promote cell survival, Akt1 has been implicated as a major factor in many types of cancer. Akt (now also called Akt1) was originally identified as the oncogenein the transforming retrovirus, AKT8.

Description:
PKAkt1 is a glycosilated polypeptide having a molecular mass of 59.1 kDa, fused with a polyhistidine tag at N-terminus (to facilitate removal of Akt1 kinase from the reaction mixture).
Inactive enzyme, suitable for negative control experiments or for phosphorylation as a substrate.
Recombinant Protein Kinase B is purified by proprietary chromatographic techniques.

Source:
Sf9 insect cells.

Physical Appearance:
Sterile Filtered clear solution.

Formulation:
PKAkt1 1.9mg/ml, in 50mM Tris-HCl, 100mM NaCl, 1mM DTT, 25mM beta glycerophosphate, 50% glycerol, pH 8.5.

Storage Conditions:
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

Purity:
Greater than 90% as determined by SDS-PAGE.

Unit definition:
1 Unit is defined as 1 picomole phosphate transferred to the synthetic peptide (RPRAATF) per min at 30°C.

Activity:
No protease activity (Twinning test).
The Specific activity is 235 U/mg.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.