产品编号:PKA-329-1ug

产品描述:Recombinant Human PI3KgHis(PI3KgHis)

反应种属:

实验方法:

标记:

规格:1µg

供应商:Prospec

价格(RMB):2800.00

订购:

说明书:

PI3KgHis Human
Phosphoinositide 3-kinase p110g Human Recombinant
Catalog No. PKA-329

Synonyms:
Phosphoinositide 3-kinase p110g, PI3Kg.

Introduction:
PI3Kg is highly expressed in cells of hematopoietic origin. It plays an important role in dendritic cell (DC) trafficking and in the activation of specific immunity. PI3Kg-/- mice showed a reduced ability to respond to chemokines, and had a selective defect in the number of skin Langerhans cells and in lymph node CD8a-DCs. In macrophages, the chemokine RANTES/CCL5 activates the small GTPase Rac1 and its downstream target PAK2. This response depends on Gi activation and largely on the subsequent triggering of PI3Kg.

Description:
Recombinant full length PI3Kg (126.3 kDa without Tag) carries an N-terminal His 8 affinity Tag.

Source:
Sf9 insect cells.

Physical Appearance:
Sterile Filtered clear solution.

Formulation:
0.5 mg/ml solution in 25mM HEPES, pH 8.0, 25mM NaCl, 2.5mM MgCl2, and 50% glycerol.

Stability:
PI3Kg although stable at 14ºC for 1 week, should be stored desiccated below -18ºC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 95.0% as determined by SDS-PAGE.

Biological Activity:
~ 4.5 nmol/mg/min using phosphatidylinositol as the substrate.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.