产品编号:ENZ-305-5ug

产品描述:Recombinant Human PL12(PL12)

反应种属:

实验方法:

标记:

规格:5µg

供应商:Prospec

价格(RMB):800.00

订购:

说明书:

PL12 Human
Alanyl-tRNA Synthetase Human Recombinant
Catalog No. ENZ-305

Synonyms:
Alanyl-tRNA synthetase cytoplasmic, EC 6.1.1.7, Alanine-tRNA ligase, AlaRS, Renal carcinoma antigen NY-REN-42, PL-12, AARS.

Introduction:
Alanyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 108 kDa protein is an autoantigen recognized by PL-12 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that epitope spreading occurs in the autoimmune PL-12 response such that even antibodies to an isolated alanyl-tRNA molecule can develop.

Description:
Alanyl-tRNA synthetase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 107,598 Dalton.
PL-12 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Source:
Sf9 insect cells.

Physical Appearance:
Sterile Filtered clear solution.

Formulation:
PL-12 is supplied in16mM HEPES buffer pH-8, 250mM sodium chloride, and 20% glycerol.

Immunological functions:
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test).

Applications:
Western-Blot with monoclonal anti-hexa-His-tag antibody & Polymyositis sera.

Coating concentration:
0.3-0.8 µg/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.

Storage Conditions:
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

Purity:
Greater than 90% as determined by SDS-PAGE.
 
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.