产品编号:ENZ-279-30mg

产品描述:Recombinant Lactate Dehydrogenase(LDH)

反应种属:

实验方法:

标记:

规格:30mg

供应商:Prospec

价格(RMB):13720.00

订购:

说明书:

LDH
Lactate Dehydrogenase Recombinant
Catalog No. ENZ-279

Synonyms:
Lactate Dehydrogenase, LDH.

Introduction:
Lactate dehydrogenase (LDH) is an enzyme(EC1.1.1.27) present in a wide variety of organisms, including plants and animals.
A tetrameric enzyme that catalyses the interconversion of pyruvateand lactatewith concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.

Description:
The DNA encoding chicken LDH-B is cloned from cDNA library of chicken heart.

Source:
Escherichia Coli.

Physical Appearance:
Sterile lyophilized powder.

Formulation:
The protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate.

Solubility:
It is recommended to reconstitute the lyophilized LDH in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Biological Activity:
The specific activity was found to be 258 U/mg protein.

Unit Definition:
One unit is defined as 1μmol of NAD+ production per minute under the assay conditions (25°C, pH 7.0). Both transaminase activities include α-hydroxyglutarate dehydrogenase activity

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.