产品编号:ENZ-351-1mg
产品描述:Recombinant Beta Lactamase(ß Lactamase)
反应种属:
实验方法:
标记:
规格:1mg
供应商:Prospec
价格(RMB):1820.00
订购:
说明书:
β Lactamase
Beta Lactamase Recombinant
Catalog No. ENZ-351
Synonyms:
β-Lactamase, EC 3.5.2.6, Cephalosporinase.
Introduction:
Beta-lactamase is a type of enzyme(EC3.5.2.6) produced by some bacteria that is responsible for their resistanceto beta-lactam antibioticslike penicillins, cephalosporins, cephamycinsand carbapenems. These antibioticshave a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties.
Description:
Beta-Lactamase Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 263 amino acids and having a molecular mass of 29 kDa.
Beta Lactamase is purified by proprietary chromatographic techniques.
Source:
Escherichia Coli.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
Lyophilized from a concentrated (1 mg/ml) solution in water containing 20mM Phosphate buffer pH-7.
Solubility:
It is recommended to reconstitute the lyophilized Beta Lactamase in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Beta Lactamase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Beta Lactamase Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity:
Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence:
MHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVRELCSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW
Unit Definition:
One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 ° C. The International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.
Specific Activity:
1,500IU/mg.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
