产品编号:1878-1

产品描述:Alpha-Tubulin antibody

反应种属:Human, Mouse, Rat

实验方法:WB, IHC, ICC, IP

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2500.00

订购:

说明书:

Alpha-Tubulin antibody

Cat.#: 1878-1

Rabbit Monoclonal Antibody

Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis. It consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). For both type of tubulin, the carboxy-terminal tail is post-translationally modified to regulate associated protein binding at microtubule surface (3). A mutation on alpha tubulin has been linked to abnormal neuronal migration and neurodevelopmental disorders in humans (4).

Recommended Applications

WB, IHC, ICC, FC, IP

Applications and Recommended Dilution Factors

WB: 1:1,000-10,000
IHC: 1:250 - 500
ICC: 1:250 - 500
FC: 1:10 - 100
IP: 1:10 - 100

Species Reactivity

Human, Mouse, Rat

Products Data

A. Western blot analysis on HeLa cell lysate using anti- alpha-Tubulin RabMAb (cat. # 1878-1), dilution 1:50,000.

B. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using anti- alpha-Tubulin RabMAb (cat# 1878-1).

C. Immunofluorescent staining of HeLa cells using anti- alpha-Tubulin RabMAb (cat# 1878-1)

Specificity

A synthetic peptide corresponding to residues near the N-term of human α-Tubulin was used as immunogen.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Alternative Names

TUBA1B, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain;Alpha-tubulin ubiquitous;Tubulin K-alpha-1

Description References

1. Wang HW et al. Nature 435: 911-915, 2005.
2. Cleveland DW et al. Cell 20: 95-105, 1980.
3. Westermann S, Weber K, Nat. Rev. Mol. Cell Biol. 4:938 -947, 2003.
4. Keays D, et al. Cell 128:45-47, 2007.

Product References

1. Gendron TF, McCartney S, Causevic E, et al, Ethanol enhances tau accumulation in neuroblastoma cells that inducibly express tau ,Neuroscience Letters 443 (2008) 67-71
[Application: WB]
2. Wang XL, Su B, Siedlaka SL, et al, Amyloid-β overproduction causes abnormal mitochondrial dynamics via differential modulation of mitochondrial fission/fusion proteins ,PNAS 105 (2008) 19318 - 19323
[Application: WB, ICC]
3. Wang XL, Su B, Lee HG, et al, Impaired Balance of Mitochondrial Fission and Fusion in Alzheimer's Disease ,J. Neurosci. 29 (2009) 9090 - 9103
4. Ko LW, Ko HHC, Lin WL, et al, Aggregates Assembled From Overexpression of Wild-Type α-Synuclein are not Toxic to Human Neuronal Cells ,J Neuropathol Exp Neurol 67 (2008) 1084-1096
[Application: WB]