产品编号:2118-1

产品描述:Akt1 Phospho (pS473) antibody

反应种属:Human, Mouse

实验方法:WB, IHC, ICC

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2850.00

订购:

说明书:

Akt1 Phospho (pS473) antibody

Cat.#: 2118-1

Rabbit Monoclonal Antibody

Akt1/PKBa is a Ser/Thr protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth . Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4). Three mammalian Akt1/PKBa isoforms have been identified.

Recommended Applications

WB, IHC, ICC

Applications and Recommended Dilution Factors

WB: 1:5000 - 10,000
IHC: 1:100 - 250
ICC: 1:100 - 250

Species Reactivity

Human, Mouse

Products Data

A. Immunohistochemical analysis of untreated cervical carcinoma using anti-Phospho-Akt1 (pS473) RabMAb (cat. # 2118-1).

B. Immunohistochemical analysis of Phosphatase- treated cervical carcinoma using anti-Phospho-Akt1 (pS473) RabMAb (cat. # 2118-1).

C. Western blot analysis on 3T3 cell lysates using anti-Phospho-Akt1 (pS473) RabMAb (cat. #2118-1), 1:10,000 dilution. Cells were either (A) untreated or (B) treated with PDGF.

Specificity

A phospho specific peptide corresponding to residues surrounding Ser473 of human Akt1 was used as immunogen. The antibody detects Akt1 phosphorylated at Serine 473.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Alternative Names

AKT1, PKB, RAC, RAC-alpha serine/threonine-protein kinase, C-AKT

Description References

1. Burgering, B.M. and P.J. Coffer.Nature 376: 599
2. Cardone, M.H., et al. Science 282: 1318
3. Hajduch, E., et al. FEBS Lett. 492: 199
4. Cross, D.A., et al. Nature 378: 785-789 (1995).

Product References

1. Renner O, Blanco-Aparicio C, Grassow M, et al, Activation of Phosphatidylinositol 3-Kinase by Membrane Localization of p110α Predisposes Mammary Glands to Neoplastic Transformation ,Cancer Res 68 (2008) 9643-9653
[Application: IHC]
2. Blanco-Aparicio C, Cañamero M, Cecilia Y, et al, Exploring the Gain of Function Contribution of AKT to Mammary Tumorigenesis in Mouse Models ,PLoS One 5 (2010) e9305
[Application: IHC]
3. Pommier AJ, Alves G, Viennois E, et al, Liver X Receptor activation downregulates AKT survival signaling in lipid rafts and induces apoptosis of prostate cancer cells ,Oncogene 29 (2010) 2712-2723
[Application: ICC]