产品编号:1565-1

产品描述:Amyloid Beta A4 Precursor (APP) antibody

反应种属:Human, Mouse, Rat

实验方法:WB, IHC, ICC, FC, IP

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2500.00

订购:

说明书:

Amyloid Beta A4 Precursor (APP) antibody

Cat.#: 1565-1

Rabbit Monoclonal Antibody

Alzheimer's disease (AD) is characterized by the presence of senile plaques in the brain and blood vessel walls. Amyloid Beta A4 (Aβ4) protein has been found to be the principal constituent of senile plaques of ADs patients (1-2). The amyloid beta-protein precursor (APP) is proteolytically cleaved to generate the 4 kDa Aβ4 protein. Two variants of Aβ4 can be detected; Aβ1-40 short-tailed and Aβ1-42 long-tailed (3-4).

Recommended Applications

WB, IHC, ICC, FC, IP

Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
IHC: 1:250-500
ICC: 1:250-500
FC: 1:20
IP: 1:50

Species Reactivity

Human, Mouse, Rat

Products Data

A. Western blot analysis on Hela cell lysate using anti- Amyloid Beta A4 precusor RabMAb (cat. # 1565-1), dilution 1:20,000

Staining of various paraffin-embedded human tissue samples using anti-Amyloid Beta A4 precursor RabMAb (cat#1565-1). Liver showed negative staining. Tissues were stained in parallel on the same Normal Tissue Array.

Staining of various paraffin-embedded human tissue samples using anti-Amyloid Beta A4 precursor RabMAb (cat#1565-1). Skeletal muscle showed negative staining. Tissues were stained in parallel on the same Normal Tissue Array.

Staining of various paraffin-embedded human tissue samples using anti-Amyloid Beta A4 precursor RabMAb (cat#1565-1). Human brain tissue showed positive immunohistochemical staining for Amyloid Beta A4 precursor. Tissues were stained in parallel on the same Normal Tissue Array.

Staining of various paraffin-embedded human tissue samples using anti-Amyloid Beta A4 precursor RabMAb (cat#1565-1). Human breast showed negative staining. Tissues were stained in parallel on the same Normal Tissue Array.

  

Specificity

A synthetic peptide corresponding to residues in the NPXY motif of human Amyloid Beta A4 precusor (APP) was used as immunogen. This antibody specifically recognizes APP and its isoforms. It will not recognize either of the two Amyloid Beta Protein ( Aβ4 ) variants, Aβ1 -40 or Aβ1 -42.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Alternative Names

APP, A4, AD1, C31, Gamma-CTF(50);Amyloid intracellular domain 50

Description References

1. Glenner, G. G., Wong, C. W. (1984) Biochem. Biophys. Res. Commun. 120, 885-890
2. Masters, C. L., Simms, G., Weinman, N. A., Multhaup, G., McDonald, B., Beyreuther, K. (1985) Proc. Natl. Acad. Sci. U. S. A. 82, 4245-4249
3. Dovey, H. F., Suomesaari-Chrysler, S., Lieberburg, I., Sinha, S., and Kiem, P. S. (1993) NeuroReport 4, 1039-1042
4. Vigo-Pelfrey, C., Lee, D., Keim, P., Lieberburg, I., Schenk, D. B. (1993) J. Neurochem. 61, 1965-1968

Product References

1. Wang ZL, Wang BP, Yang L, et al, Presynaptic and Postsynaptic Interaction of the Amyloid Precursor Protein Promotes Peripheral and Central Synaptogenesis ,J. Neurosci. 29 (2009) 10788 - 10801