产品编号:1569-1

产品描述:AMPK gamma-1(N-term)antibody

反应种属:Human

实验方法:WB, IHC, ICC, FC, IP

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2500.00

订购:

说明书:

AMPK gamma-1(N-term)antibody

Cat.#: 1569-1

Rabbit Monoclonal Antibody

The 5-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose nonfermentor) kinase family (1), is a heterotrimeric protein comprise of α (63 kDa), β (30 kDa) and γ (38 kDa) subunits. The α subunit is the catalytic subunit, while β and γ are noncatalytic subunits (although they have been found to interact with the active subunit in liver). AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (2). AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro (3).

Recommended Applications

WB, IHC, ICC, FC, IP

Applications and Recommended Dilution Factors

WB: 1:1,000-2,000
IHC: 1:250-500
ICC: 1:50-100
FC: 1:100
IP: 1:50

Species Reactivity

Human

Products Data

A. Western blot analysis of anti- AMPK gamma-1 (N-term) RabMAb (cat. # 1569-1), dilution 1:2,000. A: Jurkat B: Hela

B. Immunohistochemical analysis of paraffin-embedded human skeletal muscles using anti-AMPK gamma-1 (N-term) RabMAb (cat. # 1569-1).

  

Specificity

A synthetic peptide corresponding to residues in the N-term of human AMPK gamma-1 subunits was used as an immunogen

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Alternative Names

PRKAG1, 5'-AMP-activated protein kinase subunit gamma-1

Description References

1. Mitchelhill, K. I., Stapleton, D., Gao, G., House, C., Michell, B., Katsis, F., Witters, L. A., and Kemp, B. E. (1994) J. Biol. Chem. 269, 2361-2364
2. Carling, D., Clarke, P. R., Zammit, V. A., and Hardie, D. G. (1989) Eur. J. Biochem. 186, 129-136
3. Carling, D., Zammit, V. A,, and Hardie, D. G. (1987) FEES Lett. 223,217-222