产品编号:1592-1
产品描述:AMPK gamma-1(C-term)antibody
反应种属:Human, Mouse, Rat
实验方法:WB, FC, IP
标记:
规格:100ul
供应商:Epitomics
价格(RMB):2500.00
订购:
说明书:
Cat.#: 1592-1
The 5-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose nonfermentor) kinase family (1) AMPK is a heterotrimeric protein comprising α (63 kDa), β (38 kDa) and γ (38 kDa) subunits. The alpha subunit is the catalytic subunit, while beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (2). AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro (3).
WB, FC, IP
WB: 1:1,000
FC: 1:20
IP: 1:50
Human, Mouse, Rat
A synthetic peptide corresponding to residues in the C-term of human AMPK gamma-1 subunit was used as an immunogen
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
PRKAG1, 5'-AMP-activated protein kinase subunit gamma-1
1. Mitchelhill, K. I., Stapleton, D., Gao, G., House, C., Michell, B., Katsis, F., Witters, L. A., and Kemp, B. E. (1994) J. Biol. Chem. 269, 2361-2364
2. Carling, D., Clarke, P. R., Zammit, V. A., and Hardie, D. G. (1989) Eur. J. Biochem. 186, 129-136
3. Carling, D., Zammit, V. A,, and Hardie, D. G. (1987) FEES Lett. 223,217-222
1. Anderson KA, Ribar TJ, Lin F, et al, Hypothalamic CaMKK2 Contributes to the Regulation of Energy Balance ,Cell Metab 7 (2008) 377-388
[Application: WB]
