产品编号:1604-1

产品描述:AMPK beta 1 antibody

反应种属:Human, Mouse, Rat

实验方法:WB, IHC, FC, IP

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2500.00

订购:

说明书:

AMPK beta 1 antibody

Cat.#: 1604-1

Rabbit Monoclonal Antibody

The 5-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose nonfermentor) kinase family (1). AMPK is a heterotrimeric protein comprising α (63 kDa), β (38 kDa) and γ (38 kDa) subunits (2). The alpha subunit is the catalytic subunit, while beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (3). AMPK beta-1 mediates the association of the AMPK heterotrimeric complex in vitro (2). Two isoforms have been found and the difference in expression patterns indicates tissue-specific roles for these isoforms (4).

Recommended Applications

WB, IHC, FC, IP

Applications and Recommended Dilution Factors

WB: 1:5,000
IHC: 1:100-250
FC: 1:50
IP: 1:80

Species Reactivity

Human, Mouse, Rat

Products Data

A. Western blot analysis of anti-AMPK beta-1 RabMAb (cat. # 1604-1), dilution 1:5,000. A-NIH 3T3 B: Hela C: A431 D: PC12

B. Immunohistochemical analysis of paraffin-embedded human lung carcinoma using anti-AMPK beta-1 RabMAb (cat. # 1604-1).

  

Specificity

A synthetic peptide corresponding to residues in the human AMPK beta-1 subunit was used as an immunogen.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Alternative Names

PRKAB1, AMPK, 5'-AMP-activated protein kinase subunit beta-1

Description References

1. Mitchelhill, K. I., Stapleton, D., Gao, G., House, C., Michell, B., Katsis, F., Witters, L. A., and Kemp, B. E. (1994) J. Biol. Chem. 269, 2361-2364
2. Woods, A.; Cheung, P. C. F.; Smith, F. C.; Davison, M. D.; Scott, J.; Beri, R. K.; Carling, D.: J. Biol. Chem. 271: 10282-10290, 1996.
3. Carling, D., Clarke, P. R., Zammit, V. A., and Hardie, D. G. (1989) Eur. J. Biochem. 186, 129-136
4. Thornton, C.; Snowden, M. A.; Carling, D.: J. Biol. Chem. 273: 12443-12450, 1998.

Product References

1. Bendayan M, Londono I, Kemp BE, et al, Association of AMP-activated Protein Kinase Subunits With Glycogen Particles as Revealed In Situ by Immuno-electron Microscopy ,J. Histochem. Cytochem. 57 (2009) 963 - 971
[Application: WB]
2. Anderson KA, Ribar TJ, Lin F, et al, Hypothalamic CaMKK2 Contributes to the Regulation of Energy Balance ,Cell Metab 7 (2008) 377-388
[Application: WB]
3. Lessard SJ, Rivas DA, Chen ZP, et al, Impaired Skeletal Muscle β-Adrenergic Activation and Lipolysis Are Associated with Whole-Body Insulin Resistance in Rats Bred for Low Intrinsic Exercise Capacity ,Endocrinology 150 (2009) 4883 - 4891
[Application: WB]
4. Dzamko N, van Denderen BJ, Hevener AL, et al, AMPK 1 Deletion Reduces Appetite, Preventing Obesity and Hepatic Insulin Resistance ,J Biol Chem 285 (2010) 115-122
[Application: WB]
5. Bendayan M, Londono I, Kemp BE, et al, Association of AMP-activated Protein Kinase Subunits With Glycogen Particles as Revealed In Situ by Immunoelectron Microscopy ,J. Histochem. Cytochem. 57 (2009) 963 - 971