产品编号:2084-1

产品描述:Amphiphysin antibody

反应种属:Human, Mouse, Rat

实验方法:WB, IHC, ICC, FC

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2500.00

订购:

说明书:

Amphiphysin antibody

Cat.#: 2084-1

Rabbit Monoclonal Antibody

Amphiphysin, a neuronal protein first identified in chicken synaptic membranes, is the autoantigen of Stiff-Man Syndrome (SMS) associated with breast cancer. Patient autoantibodies have a distinct pattern of reactivity with amphiphysin, and the dominant autoepitope is located in its C-terminal region, which contains an SH3 domain (1). Amphiphysin is expressed in many neurons, certain endocrine cell types, and spermatocytes (2). study suggests a link between amphiphysin I expression in cancer and amphiphysin I autoimmunity. The enhanced expression of amphiphysin I in some forms of cancer supports the hypothesis that amphiphysin family members may play a role in the biology of cancer cells (3).

Recommended Applications

WB, IHC, ICC, FC

Applications and Recommended Dilution Factors

WB: 1:25,000 - 50,000
IHC: 1:100 - 250
ICC: 1:100 - 250
FC: 1:15

Species Reactivity

Human, Mouse, Rat

Products Data

A. Western blot analysis on human brain lysate using anti-Amphipysin RabMAb (cat. # 2084-1), dilution 1:100,000.

B. Immunohistochemical analysis of paraffin-embedded human brain using anti-Amphiphysin RabMAb (cat. #2084-1).

C. Immunofluorescent staining of SH-SYS5 cells using anti-Amphiphysin RabMAb (cat. #2084-1).

Specificity

A synthetic peptide corresponding to residues within human Amphipysin was used as an immunogen.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Description References

1. David C, et al. FEBS Lett 351(1):73-9, 1994
2. Yamamoto R, et al. Hum Mol Genet 4(2):265-8, 1995
3. Floyd S, et al. Mol Med 4(1):29-39, 1998