产品编号:2316-1
产品描述:Alpha-Actinin 3 antibody
反应种属:Human, Mouse, Rat
实验方法:WB, IHC, IC, FC
标记:
规格:100ul
供应商:Epitomics
价格(RMB):2500.00
订购:
说明书:
Cat.#: 2316-1
The alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibres, while ACTN3 expression is restricted to a subset of type 2 fibers. Absence of alpha-actinin-3 is not associated with an obvious disease phenotype, raising the possibility that ACTN3 is functionally redundant in humans, and that alpha-actinin-2 is able to compensate for alpha-actinin-3 deficiency (1). Alpha-Actinin-3 is specifically expressed in fast-twitch myofibers responsible for generating force at high velocity. The absence of a disease phenotype secondary to alpha-actinin-3 deficiency is likely due to compensation by the homologous protein, alpha-actinin-2. However, the high degree of evolutionary conservation of ACTN3 suggests function(s) independent of ACTN2. Highly significant associations between ACTN3 genotype and athletic performance have been demonstrated in both male and female athletes.
WB, IHC, ICC, FC
WB: 1:5,000-10,000
IHC: 1:100 - 250
ICC: 1:100 - 250
FC: 1:200
Human, Mouse, Rat
A synthetic peptide corresponding to residues on the N-terminus of human alpha-actinin 3 was used as an immunogen.
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months.
1. Mills M, et al. Hum Mol Genet 10(13):1335-46, 2001 2. Yang N, et al. Am J Hum Genet 73(3):627-31, 2003
