产品编号:2507-1

产品描述:Alpha Fodrin antibody

反应种属:Human, Mouse, Rat

实验方法:WB, IHC, ICC

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2500.00

订购:

说明书:

Alpha Fodrin antibody

Cat.#: 2507-1

Rabbit Monoclonal Antibody

Alpha Fodrin is a member of the spectrin family of widely distributed filamentous proteins that form a supporting and organizing scaffold for cell membranes (1). Non-erythroid alpha spectrin, also called Alpha Fodrin, is a major cortical cytoskeletal protein that has been implicated in the establishment of specialized membrane- cytoskeletal domains in differentiating cells (2-3). Cleavage of Alpha Fodrin accompanies apoptosis, induced by activation via the CD3/T cell receptor complex, ligation of the Fas (CD95) molecule and other Fas-expressing cells, or treatment of cells with staurosporine, dexamethasone, or synthetic ceramide (4). The major products of the cleavage are amino-terminal (135 kDa) and carboxy-terminal (150 kDa and 120 kDa) fragments. Antibodies against Alpha Fodrin are observed in diseases characterized by chronic apoptosis, and are valuable in the evaluation fo Sicca- and Sjögren's syndrome (5-6).

Recommended Applications

WB, ICC

Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
ICC: 1:100 - 250

Species Reactivity

Human, Mouse, Rat

Products Data

A. Western blot analysis on (A) HeLa treated with Camptothecin, (B) HeLa, (C) Jurkat treated with Staurosporine and (D) Jurkat cell lysates using anti-alpha Fodrin RabMAb (cat. # 2507-1), dilution 1:1000.

B. Western blot analysis on (A) TF-1, (B) SH-SY5Y, (C) HeLa and (D) human brain lysates using anti-alpha Fodrin RabMAb (cat. # 2507-1), dilution 1:2000.

  

Specificity

A synthetic peptide corresponding to residues in human Alpha Fodrin was used as an immunogen.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Alternative Names

SPTAN1, SPTA2, Spectrin alpha chain, brain, Spectrin, non-erythroid alpha chain;Alpha-II spectrin;Fodrin alpha chain

Description References

1. Leto TL, et al. Mol Cell Biol. 8(1):1-9, 1988
2. Simonovic M, et al. J Biol Chem. 281(45):34333-40, 2006
3. DH Giebelhaus, et al. The Journal of Cell Biology 105:843-853,1987
4. Martin SJ, et al. J Biol Chem. 270(12):6425-8, 1995
5. Ulbricht K U, et al. Autoimmunity Reviews 2(2):109-113,2003
6. Kahaly, G. J., et al. Clinical & Experimental Immunology 140:166-172(7), 2005