产品编号:1891-1
产品描述:Alk Phospho (pY1604) antibody
反应种属:Human
实验方法:WB, IHC, IP
标记:
规格:100ul
供应商:Epitomics
价格(RMB):2850.00
订购:
说明书:
Cat.#: 1891-1
ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. The 2;5 chromosomal translocation occurs in most anaplastic large-cell non-Hodgkin's lymphomas arising from activated T lymphocytes. This rearrangement was shown to fuse the NPM nucleolar phosphoprotein gene on chromosome 5q35 to a previously unidentified protein tyrosine kinase gene, ALK, on chromosome 2p23 (1). In the predicted hybrid protein, the amino terminus of NPM is linked to the catalytic domain of ALK. Immunoblotting with anti-ALK antibody shows that ALK is highly expressed in the neonatal brain (2). Also expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. Unscheduled expression of the truncated ALK may contribute to malignant transformation in these lymphomas (3).
WB, IHC, IP
WB: 1:5000
IHC: 1:100 - 250
IP: 1:20
Human
A synthetic phosphor-peptide corresponding to residues surrounding Tyr1604 of human ALK was used as an immunogen. The antibody only detects ALK phosphorylated on Tyrosine 1604.
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
ALK, ALK tyrosine kinase receptor, Anaplastic lymphoma kinase
1. Morris SW, et al. Science 263(5151):1281-4, 1994.
2. Iwahara T, et al. Oncogene 14(4):439-49, 1997.
3. Benharroch D, et al. Blood 91(6): 2076-84, 1998.
