产品编号:2483-1

产品描述:Aldolase B antibody

反应种属:Human, Mouse, Rat

实验方法:WB, IP

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2500.00

订购:

说明书:

Aldolase B antibody

Cat.#: 2483-1

Rabbit Monoclonal Antibody

Aldolase B is one of three known aldolase isoenzymes, and is found in kidney and small adult intestine where it is associated with aldolases A or C. Aldolase B catalyzes the reversible cleavage of fructose 1-phosphate into dihydroxyacetone phosphate and glyceraldehyde. It is involved in both glycolytic and gluconeogenic pathways, where its quaternary structure is critical for maintaining its full catalytic function. Hereditary fructose intolerance (HFI), a potentially fatal human autosomal recessive disease of carbohydrate metabolism, results with Aldolase B deficiency. Partially active Aldolase B, however, has been reported in an HFI individuals, which supports the hypothesis that adequate gluconeogenesis/glycolysis is maintained in HFI patients by the presence of partially active enzymes (1-4).

Recommended Applications

WB, IHC, FC

Applications and Recommended Dilution Factors

WB: 1:500 - 1000
IHC: 1:100 - 250
FC: 1:20

Species Reactivity

Human, Mouse, Rat

Products Data

A. Western blot analysis on fetal kidney lysate using anti-Aldolase B RabMAb (cat. # 2483-1), dilution 1:1000.

B. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Adolase B RabMAb (cat. #2483-1).

  

Specificity

A synthetic peptide corresponding to residues in human Aldolase B was used as an immunogen.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Alternative Names

ALDOB, ALDB, Fructose-bisphosphate aldolase B, Liver-type aldolase

Description References

1. Rellos P, et al. J Biol Chem. 275(2):1145-51, 2000
2. Cross NC, et al. Cell. 53(6):881-5, 1988
3. Brooks CC, et al. FASEB J. 8(1):107-13, 1994
4. Gregori C, et al. J Biol Chem, 273(39):25237-25243, 1998