产品编号:2023-1

产品描述:Aldh1A1 antibody

反应种属:Human

实验方法:WB, IHC, ICC, IP

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2500.00

订购:

说明书:

Aldh1A1 antibody

Cat.#: 2023-1

Rabbit Polyclonal IgG Affinity Purified

Usual human livers contain two major ALDH isozymes, i.e., cytosolic ALDH1 and mitochondrial ALDH2(1). Human red cell aldehyde dehydrogenase (ALDH) resembles the liver cytosolic isozyme in numerous physicochemical properties. Thus, the erythrocyte enzyme, by virtue of its chemical and structural identity with the liver cytosolic enzyme, may serve as a suitable peripheral enzyme model to understand the cause and mechanism of alcohol abuse-related changes in liver cytosolic ALDH that has been found to be reduced in alcoholics (2). ALDH1A1 is known to catalyze the oxidation of retinaldehyde to retinoic acid. Overexpression of human ALDH1A1 in HEK cells led to a more than 20-fold increase in 3-deoxyglucosone dehydrogenase activity (3).

Recommended Applications

WB, IHC, ICC, IP

Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
IHC: 1:100 - 250
ICC: 1:50 - 100
IP: 1:40

Species Reactivity

Human

Products Data

A. Western blot analysis on fetal liver lysate of using anti-ALDH1A1 Rabbit Polyclonal (cat. # 2023-1), dilution 1:2000.

B. Immunohistochemical analysis of paraffin-embedded liver using anti-ALDH1A1 Rabbit Polyclonal (cat. #2023-1).

  

Specificity

A synthetic peptide corresponding to residues within human ALDH1A1 was used as an immunogen.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Description References

1. Yoshida A, et al. Alcohol 2(1):103-6, 1985
2. Agarwal DP, et al. Enzyme 42(1):47-52, 1989
3. Collard F, et al. Biochimie 89(3):369-73, 2007