产品编号:2214-1

产品描述:Akt1 Phospho (pT308) antibody

反应种属:Human, Mouse

实验方法:WB

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2850.00

订购:

说明书:

Akt1 Phospho (pT308) antibody

Cat.#: 2214-1

Rabbit Monoclonal Antibody

Akt1/PKBa is a Ser/Thr protein kinase, whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth. Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3, 4). Three mammalian Akt1/PKBa isoforms have been identified.

Recommended Applications

WB

Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000

Species Reactivity

Human, Mouse

Products Data

A. Western blot analysis on NIH 3T3 cell lysates using anti-Phospho-Akt1 (pT308) RabMAb (cat. #2214-1), 1:10,000 dilution. Cells were either (A) untreated (B) treated with PDGF

  

  

Specificity

A phospho specific peptide corresponding to residues surrounding Threonine 308 of human Akt1 was used as immunogen. The antibody detects Akt1 phosphorylated at Threonine 308.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Alternative Names

AKT1, PKB, RAC, RAC-alpha serine/threonine-protein kinase, C-AKT

Description References

1. Burgering, B.M. and P.J. Coffer.Nature 376: 599
2. Cardone, M.H., et al. Science 282: 1318
3. Hajduch, E., et al. FEBS Lett. 492: 199
4. Cross, D.A., et al. Nature 378: 785-789, 1995.

Product References

1. Feng Q, Di R, Tao F, et al, PDK1 Regulates Vascular Remodeling and Promotes Epithelial-Mesenchymal Transition in Cardiac Development ,Mol Cell Biol 30 (2010) 3711-3721