产品编号:1086-1
产品描述:Akt1 antibody
反应种属:Human, Mouse, Rat
实验方法:WB
标记:
规格:100ul
供应商:Epitomics
价格(RMB):2100.00
订购:
说明书:
Akt1/PKBa is a Ser/Thr protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth. Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4). Three mammalian Akt1/PKBa isoforms have been identified.
WB
WB: 1:10,000
Human, Mouse, Rat
A synthetic peptide corresponding to residues surrounding Ser473 of human Akt1 was used as immunogen. The antibody can detect Akt1 and Akt3. Predicted to cross-react with mouse, rat and bovine, based on sequence homology.
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
AKT1, PKB, RAC, RAC-alpha serine/threonine-protein kinase, C-AKT
1. Burgering, B.M. and P.J. Coffer. Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction. Nature 376: 599
2. Cardone, M.H., et al. Regulation of cell death protease caspase-9 by phosphorylation. Science 282: 1318
3. Hajduch, E., et al. Protein kinase B (PKB/Akt)--a key regulator of glucose transport? FEBS Lett. 492: 199
4. Cross, D.A., et al. Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B. Nature 378: 785-789 (1995).
