产品编号:1020-1
产品描述:AIF antibody
反应种属:Human, Mouse, Rat
实验方法:WB, IHC, ICC, IP, FC
标记:
规格:100ul
供应商:Epitomics
价格(RMB):2500.00
订购:
说明书:
Cat.#: 1020-1
AIF, or apoptosis-inducing factor, is a flavoprotein localized to the mitochondrial membrane that plays a primary role in caspase-independent cell death, or apoptosis (1,2). AIF translocates to the nucleus when apoptosis is induced and causes mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. Apoptotic activity of AIF leads to chromatin condensation and DNA fragmentation (1). Similar to cytochrome c, AIF is a bifunctional protein with an oxidoreductase function and a second apoptogenic function. In contrast to cytochrome c, however, AIF acts in a caspase-independent fashion (2,3,4).
WB, IHC, ICC, IP, FC
WB: 1:1,000
IHC: 1:500
ICC: 1:500
FC: 1:50
IP: 1:10
Human, Mouse, Rat
A synthetic peptide corresponding to residues near the C-terminus of Human AIF was used as immunogen.
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
AIFM1, AIF, PDCD8, Apoptosis-inducing factor 1, mitochondrial, Programmed cell death protein 8
1. Susin, S.A. et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 397(6718): 441
2. Daugas, E. et al. Apoptosis-inducing factor (AIF): a ubiquitous mitochondrial oxidoreductase involved in apoptosis. FEBS Lett. 476(3): 118
3. Daugas, E. et al. Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. FASEB J. 14(5):729
4. Susin, S.A. et al. Two distinct pathways leading to nuclear apoptosis. J. Exp. Med. 192(4): 571
1. Cao GD, Xing J, Xiao X, et al, Critical Role of Calpain I in Mitochondrial Release of Apoptosis-Inducing Factor in Ischemic Neuronal Injury ,The Journal of Neuroscience 27 (2007) 9278 –9293
[Application: WB]
2. Ishimura R, Martin GR and Ackerman SL, Loss of Apoptosis-Inducing Factor Results in Cell-Type-Specific Neurogenesis Defects ,The Journal of Neuroscience 28 (2008) 4938–4948
[Application: IHC]
3. Bajt ML, Farhood A, Lemasters JJ, et al, Mitochondrial Bax Translocation Accelerates DNA Fragmentation and Cell Necrosis in a Murine Model of Acetaminophen Hepatotoxicity ,JPET 324 (2008) 8–14
[Application: WB]
4. Bajt ML, Cover C, Lemasters JJ, et al, Nuclear Translocation of Endonuclease G and Apoptosis-Inducing Factor during Acetaminophen-Induced Liver Cell Injury ,Toxicologial Sciences 94 (2006) 217–225
[Application: IHC]
5. Yu SW, Andrabi SA, Wang HM, et al , Apoptosis-inducing factor mediates poly(ADP-ribose) (PAR) polymer-induced cell death ,PNAS 103 (2006) 18314 –18319
[Application: ICC]
6. Lin CY, Wu HY, Wang PL, et al, Mammalian Ste20-like protein kinase 3 induces a caspase-independent apoptotic pathway ,Int J Biochem Cell Biol 42 (2010) 98-105
[Application: WB]
7. Shen H, Hu X, Liu C, et al, Ethyl pyruvate protects against hypoxic-ischemic brain injury via anti-cell death and anti-inflammatory mechanisms ,Neurobiol Dis 37 (2010) 711-722
[Application: WB]
8. Chen X, Gong J, Zeng H, et al, MicroRNA145 Targets BNIP3 and Suppresses Prostate Cancer Progression ,Cancer Res 70 (2010) 2728-2738
9. Saito C, Lemasters JJ, Jaeschke H, c-Jun N-terminal kinase modulates oxidant stress and peroxynitrite formation independent of inducible nitric oxide synthase in acetaminophen hepatotoxicity ,Toxicol Appl Pharmacol 246 (2010) 8-17
[Application: WB]
10. Huang R, Chen XQ, Huang Y, et al, The multikinase inhibitor sorafenib induces caspase-dependent apoptosis in PC-3 prostate cancer cells ,Asian J Androl 12 (2010) 527-534
[Application: WB]
