产品编号:2208-1

产品描述:Adducin alpha Phospho (pS59) antibody

反应种属:Human

实验方法:WB, IP

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2850.00

订购:

说明书:

Adducin alpha Phospho (pS59) antibody

Cat.#: 2208-1

Rabbit Monoclonal Antibody

Alpha adducin is a 80-120 kD member of the aldolase class II family and adducin subfamily. Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; α and β, or α and γ subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). PKA specifically phosphorylates Ser59, Ser408, Ser436, and Ser481 located in the neck domain of alpha Adducin. Phosphorylation of Adducin alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of Adducin alpha (5).

Recommended Applications

WB, IP

Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
IP: 1:40

Species Reactivity

Human

Products Data

A. Western blot analysis on HeLa cell lysates using anti-Phospho-Adducin alpha (pS59) RabMAb (cat. #2208-1), 1:10,000 dilution. Cells were either (A) untreated (B) treated with calyculin A

  

  

Specificity

A synthetic phospho-peptide corresponding to residues surrounding Ser59 of human alpha Adducin was used as an immunogen. The antibody only detects alpha Adducin phosphorylated on Serine 59.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Alternative Names

ADD1, ADDA, Alpha-adducin, Erythrocyte adducin subunit alpha

Description References

1. Gardner K, Bennett V., J Biol Chem 261:1339-1348, 1986.
2. Kuhlman PE, et al. J Biol. Chem, 271: 7986-7991, 1996.
3. Dong, LC, et al. J Biol Chem 270: 25534-25540, 1995.
4. Palfrey HC, et al. J Biol Chem. 260:16021-16029, 1985.
5. Fukata, et al. J Cell Biol, 145:347-361, 1995.