产品编号:1762-1

产品描述:Adducin alpha Phospho (pS481) antibody

反应种属:Human, Mouse, Rat

实验方法:WB, IHC, ICC, IP

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2850.00

订购:

说明书:

Adducin alpha Phospho (pS481) antibody

Cat.#: 1762-1

Rabbit Monoclonal Antibody

Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; α and β, or α and γ subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). PKA specifically phosphorylates Ser408, Ser436, and Ser481 located in the neck domain of alpha Adducin. Phosphorylation of Adducin alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of Adducin alpha (5).

Recommended Applications

WB, IHC, ICC, IP

Applications and Recommended Dilution Factors

WB: 1:1,000-10,000
IHC: 1:250 - 500
ICC: 1:100 -250
IP: 1:50

Species Reactivity

Human, Mouse, Rat

Products Data

A. Western blot analysis on (1) Hela cell lysate and (2) HeLa lysate + TPA using anti-Phospho-Adducin (pS481) RabMAb (catalog #1762-1), dilution 1:20,000.

B. Immunohistochemical analysis of paraffin-embedded human uterus using anti-Adducin alpha Rab MAb. (cat. # 1762-1).

  

Specificity

A synthetic phospho-peptide corresponding to residues surrounding Ser481 of human alpha Adducin was used as an immunogen. The antibody only detects alpha Adducin phosphorylated on Serine 481.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Alternative Names

ADD1, ADDA, Alpha-adducin, Erythrocyte adducin subunit alpha

Description References

1. Gardner K, Bennett V., J Biol Chem 261:1339-1348, 1986.
2. Kuhlman PE, et al. J Biol. Chem, 271: 7986-7991, 1996.
3. Dong, LC, et al. J Biol Chem 270: 25534-25540, 1995.
4. Palfrey HC, et al. J Biol Chem. 260:16021-16029, 1985.
5. Fukata, et al. J Cell Biol, 145:347-361, 1995.