产品编号:1706-1

产品描述:Adducin alpha(C-term)antibody

反应种属:Human, Mouse

实验方法:WB, IHC, ICC

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2500.00

订购:

说明书:

Adducin alpha(C-term)antibody

Cat.#: 1706-1

Rabbit Monoclonal Antibody

Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; α and β, or α and γ subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). Phosphorylation of adducing alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of adducing alpha (5).

Recommended Applications

WB, IHC, ICC

Applications and Recommended Dilution Factors

WB: 1:5,000-50,000
IHC: 1:250-500
ICC: 1:250-500

Species Reactivity

Human, Mouse

Products Data

A. Western blot analysis on Hela cell lysate using anti-K562 RabMAb (cat. # 1706-1), dilution 1:50,000.

B. Immunohistochemical analysis of paraffin-embedded human lung using anti-Adducin (C-term) RabMAb (cat. # 1706-1).

C. Immunofluorescent staining of HeLa using anti-Adducin RabMAb (C-term) (cat. # 1706-1).

Specificity

A synthetic peptide corresponding to residues in the C-term of human Adducin was used as immunogen.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Alternative Names

ADD1, ADDA, Alpha-adducin, Erythrocyte adducin subunit alpha

Description References

1. Gardner, K.; Bennett, V. : J. Biol. Chem. 261: 1339-1348, 1986.
2. Kuhlman, P.A., C.A. Hughes, V. Bennett, and V.M. Fowler. 1996. J. Biol. Chem. 271: 7986-7991
3. Dong, L., C. Chapline, B. Mousseau, L. Fowler, K. Ramsay, J.L. Stevens, and S. Jaken. 1995. J. Biol. Chem. 270: 25534-25540
4. Palfrey, H. C., Waseem, A. (1985) J. Biol. Chem. 260, 16021-16029
5. Fukata et al. J. Cell Biol., Volume 145, Number 2, April 19, 1999 347-361