产品编号:1844-1

产品描述:Actin antibody

反应种属:Human, Mouse, Rat

实验方法:WB, IHC, ICC, FC, IP

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2500.00

订购:

说明书:

Actin antibody

Cat.#: 1844-1

Rabbit Monoclonal Antibody

Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4).

Recommended Applications

WB, IHC, ICC, FC, IP

Applications and Recommended Dilution Factors

WB: 1:1,000
IHC: 1:100 - 200
ICC: 1:250 - 500
FC: 1:20
IP: 1:20

Species Reactivity

Human, Mouse, Rat

Products Data

A. Western blot analysis on A431 cell lysate using anti-Actin RabMAb (cat. # 1844-1), dilution 1:1,000

B. Immunohistochemical staining of paraffin-embedded human cardiac muscle using anti-Actin RabMAb (cat. # 1844-1).

C. Immunohistochemical analysis of paraffin-embedded human skeletal muscle using anti-Actin (Pan) RabMAb (Cat# 1844-1).

D. Immunofluorescent staining of HeLa cells using anti-Actin RabMAb (cat. # 1844-1).

  

  

Specificity

A synthetic peptide corresponding to residues near the N-terminus of human Actin was used as an immunogen. This Actin antibody recognizes all muscle actins.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Alternative Names

ACTC1, ACTC, Actin, alpha cardiac muscle 1, Alpha-cardiac actin

Description References

1. Herman, I.M. Curr. Opin. Cell Biol. 5: 48–55, 1993. 2. Bailly, M., et al. Curr Biol. 11: 620–5, 2001. 3. Lim, Y.P., et al. Clin. Cancer Res. 10: 3980–3987, 2004. 4. Olson, T.M., et al. Science 280: 750–2, 1998.

Product References

1. Bhattacharya R, Gonzalez AM, DeBiase PJ, et al, Recruitment of vimentin to the cell surface by β3 integrin and plectin mediates adhesion strength ,J. Cell Sci.122 (2009) 1390 - 1400
2. Wang JJ, Hu HQ, Wang S, et al, The important role of actinin-like protein (AcnA) in cytokinesis and apical dominance of hyphal cells in Aspergillus nidulans ,Microbiology 155 (2009) 2714 - 2725
3. Bhattacharya R, Gonzalez AM, Debiase PJ, et al, Recruitment of vimentin to the cell surface by β3 integrin and plectin mediates adhesion strength ,J Cell Sci 122 (2009) 1390-1400