产品编号:1912-1
产品描述:ACAT(C-term)antibody
反应种属:Human
实验方法:WB, IHC, ICC, IP
标记:
规格:100ul
供应商:Epitomics
价格(RMB):2500.00
订购:
说明书:
Cat.#: 1912-1
Triacylglycerol hydrolase is a microsomal enzyme that hydrolyzes stored cytoplasmic triacylglycerol in the liver and participates in the lipolysis/re-esterification cycle during the assembly of very-low-density lipoproteins. Expression of human TGH in Escherichia coli yields a protein without enzymatic activity, which suggests that posttranslational processing is necessary for the catalytic activity (1). ACAT esterifies free cholesterol and stores cholesteryl esters in lipid droplets. Macrophage ACAT1 deficiency results in increased atherosclerotic lesion area in hyperlipidemic mice via disrupted cholesterol efflux, increased lipoprotein uptake, accumulation of intracellular vesicles, and accelerated apoptosis (2). Data show that macrophage ACAT1 influences the efflux of both cellular and lipoprotein-derived cholesterol and propose a pathway for the pro-atherogenic transformation of ACAT1(-/-) macrophages (3).
WB, IHC, ICC, IP
WB: 1:10,000
IHC: 1: 250 - 500
ICC: 1:50 - 100
IP: 1:100
Human
A synthetic peptide corresponding to residues on the C-terminus of human ACAT was used as an immunogen.
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
CES1, CES2, SES1, Liver carboxylesterase 1, Egasyn;Retinyl ester hydrolase
1. Alam M, et al. Biochemistry 41(21):6679-87, 2002.
2. Dove DE, et al. Atherosclerosis 186(2):267-74, 2006.
3. Sone DE, et al. Arterioscler Thromb Vasc Biol 25(1): 128-34, 2005.
