产品编号:1864-1

产品描述:ACAT antibody

反应种属:Human, Mouse, Rat

实验方法:WB, IHC, ICC

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2500.00

订购:

说明书:

ACAT antibody

Cat.#: 1864-1

Rabbit Polyclonal IgG Affinity Purified

Acyl-CoA:cholesterol acyltransferase (ACAT) is an integral membrane enzyme that belongs to the type-B carboxylesterase/lipase family. ACAT catalyzes the formation of cholesteryl esters from cholesterol and long-chain fatty-acyl-coenzyme A (1). ACAT also function as a regulator of intracellular cholesterol homeostasis at a single cell level. Two isoforms of ACAT, ACAT1 and ACAT2, are expressed in the liver and intestine. However, ACAT1 is only found in macrophages and SMCs (2). The accumulation of cholesteryl esters produced by ACAT in macrophages can lead to foam cell formation, an indication of early stage atherosclerosis. ACAT activity has been linked to Alzheimer's Disease, a condition caused by excess amount of amyloid beta-peptide (Abeta). Atherogenic lipoproteins activate ACAT activity, where active ACAT can modulate the level of Abeta (3).

Recommended Applications

WB, IHC, ICC

Applications and Recommended Dilution Factors

WB: 1:500
IHC: 1:100-1:250
ICC: 1:100-1:250

Species Reactivity

Human, Mouse, Rat

Products Data

A. Western blot analysis on fetal liver using anti-ACAT RabPAb (Cat # 1864-1), dilution 1:500.

B. Immunohistochemical analysis of paraffin-embedded human liver using anti-ACAT RabPAb (Cat# 1864-1).

  

Specificity

A synthetic peptide corresponding to residues in human ACAT was used as immunogen.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Alternative Names

CES1, CES2, SES1, Liver carboxylesterase 1, Egasyn;Retinyl ester hydrolase

Description References

1. Buhman KF et al. Biochim Biophys Acta 1529(1-3):142-54, 2000