产品编号:1945-1

产品描述:ABCG1 antibody

反应种属:Human, Mouse, Rat

实验方法:WB, IHC, ICC

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2500.00

订购:

说明书:

ABCG1 antibody

Cat.#: 1945-1

Rabbit Monoclonal Antibody

ATP-binding cassette (ABC) transporter ABCG1 (ABC8) is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. Conversely, lipid efflux in cholesterol-laden macrophages, mediated by the cholesterol acceptor HDL(3), suppresses the expression of ABCG1. Immunocytochemical and flow cytometric analyses revealed that ABCG1 is expressed on the cell surface and in intracellular compartments of cholesterol-laden macrophages. Results demonstrate a regulator function for ABCG1 in cholesterol and phospholipid transport define a biologic activity for ABC transporters in macrophages (1). ABC transporters belonging to the ABCG (White) subfamily (ABCG1, ABCG5, and ABCG8) have been shown to be critically involved in the regulation of lipid-trafficking mechanisms in macrophages, hepatocytes, and intestinal mucosa cells (2) Studies show that treatment with Rhodobacter sphaeroiders LPS, an LPS antagonist, or PD169316, a specific p38 MAP kinase inhibitor, prevented the induction of ABCA1 by LPS. Therefore, this suggests that both transport of LPS from the plasma membrane to an intracellular site and activation of p38 MAP kinase are involved in the LPS-mediated induction of ABCA1 (3).

Recommended Applications

WB, IHC, ICC

Applications and Recommended Dilution Factors

WB: 1:2000
IHC: 1:50 - 100
ICC: 1:100 - 250

Species Reactivity

Human, Mouse, Rat

Products Data

A. Western blot analysis on HUVEC cell lysate using anti-ABCG1 RabMAb (cat. # 1945-1), dilution 1:2000

B. Immunohistochemical staining of paraffin-embedded human cervical carcinoma using anti-ABCG1 RabMAb (cat. #1945-1).

  

Specificity

A synthetic peptide corresponding to residues on human ABCG1 was used as an immunogen.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Alternative Names

ABCG1, ABC8, WHT1, ATP-binding cassette sub-family G member 1, ATP-binding cassette transporter 8;White protein homolog

Description References

1. Klucken J, et al. Proc Natl Acad Sci 97(2):817-22, 2000
2. Kaplan R, et al. J Lipid Res 43(6):952-9, 2002.
3. Schmitz G, et al. J Lipid Res 42(10):1513-20, 2001

Product References

1. Bogan RL, Hennebold JD, The reverse cholesterol transport system as a potential mediator of luteolysis in the primate corpus luteum ,Reproduction 139 (2010) 163-176
[Application: WB]
2. Field FJ, Watt K, Mathur SN, TNF-α decreases ABCA1 expression and attenuates HDL cholesterol efflux in the human intestinal cell line Caco-2 ,J Lipid Res 51 (2010) 1407-1415