产品编号:2411-1
产品描述:4E-BP1 Phospho(pT37)antibody
反应种属:Human, Mouse
实验方法:WB
标记:
规格:100ul
供应商:Epitomics
价格(RMB):2850.00
订购:
说明书:
Cat.#: 2411-1
4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eIF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP1. Non-phosphorylated 4E-BP1 will bind strongly to eIF4E while, the phosphorylated form will no (2). Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 70 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3). It has been reported previously that phosphorylation of 4E-BP1 on Thr 37 and Thr 46 is relatively insensitive to serum deprivation and rapamycin treatment, and that phosphorylation of these residues is required for the subsequent phosphorylation of a set of unidentified serum-responsive sites (4)
WB
WB: 1:1000 - 2000
Human, Mouse
A phospho specific peptide corresponding to residues surrounding threonine 37 was used as an immunogen. This antibody detects 4E-BP1 phosphorylated at threonine 37.
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
EIF4EBP1, Eukaryotic translation initiation factor 4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1
1. Pause, A., et al. 1994. Nature 371: 762-767
2. Gingras, A.-C., et al. Genes & Dev. 12: 502-513, 1998
3. Iritani, BM et al. (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 13180
