产品编号:2206-1

产品描述:4E-BP1 Phospho(pS65)antibody

反应种属:Human, Mouse

实验方法:WB

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2850.00

订购:

说明书:

4E-BP1 Phospho (pS65) antibody

Cat.#: 2206-1

Rabbit Monoclonal Antibody

4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 65. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4)

Recommended Applications

WB

Applications and Recommended Dilution Factors

WB: 1:2000

Species Reactivity

Human, Mouse

Products Data

A. Western blot analysis on NIH-3T3 cell lysates using anti-

  

  

Specificity

A phospho-specific peptide corresponding to residues surrounding serine 65 of human 4E-BP1 was used as an immunogen. This antibody detects 4E-BP1 phosphorylated at serine 65.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Alternative Names

EIF4EBP1, Eukaryotic translation initiation factor 4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1

Description References

1. Pause, A., 1994. Nature 371: 762-767
2. Gingras, A.-C. 1998. Genes & Dev. 12: 502-513
3. Iritani, B. M, (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 13180
4. Trumpp, A., (2001) Nature 414, 768