产品编号:1058-1

产品描述:14-3-3 b/a (N-term)antibody

反应种属:Human, Mouse, Rat

实验方法:WB, IHC, FC

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2500.00

订购:

说明书:

14-3-3 b/a (N-term) antibody

Cat.#: 1058-1

Rabbit Monoclonal Antibody

Clone ID: E58
Swiss Prot: P31946
Mol Weight: 30kDa

The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints (1). The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins (2,3). Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol (3)

Recommended Applications

WB, IHC, FC

Applications and Recommended Dilution Factors

WB: 1:1,000
IHC: 1:250
FC: 1:50
 

Species Reactivity

Human, Mouse, Rat

Products Data

A. Western blot analysis of HeLa cell lysate probed with anti-14-3-3 b/a RabMab (Cat#1058-1).

B. Immunohistochemical analysis of human skin using anti-14-3-3 b/a RabMab (cat#1058-1).

C. Flow cytometric analysis of permeabilized HeLa cells using anti-14-3-3 b/a RabMAb (red) (catalog #1058-1) or a rabbit IgG (negative) (green).

Specificity

A synthetic peptide corresponding to residues near N-terminus of human 14-3-3 beta/alpha was used as immunogen. Predicted to cross-react with sheep and bovine, based on sequence homology.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Alternative Names

YWHAB, 14-3-3 protein beta/alpha, N-terminally processed, Protein 1054

Description References

1. Fu, H., et al. 14-3-3 proteins: structure, function, and regulation. Ann. Rev. Pharmacol. Toxicol. 40: 617
2. Muslin, A.J. et al. Interaction of 14-3-3 with Signaling Proteins Is Mediated by the Recognition of Phosphoserine. Cell 84: 889
3. Zha, J. et al. Serine Phosphorylation of Death Agonist BAD in Response to Survival Factor Results in Binding to 14-3-3 Not BCL-XL. Cell 87: 619