产品编号:1062-1

产品描述:14-3-3 b/a(C-term)antibody

反应种属:Human, Mouse, Rat

实验方法:WB, IHC, ICC, FC, IP

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2500.00

订购:

说明书:

14-3-3 b/a(C-term)antibody

Cat.#: 1062-1

Rabbit Monoclonal Antibody

The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints (1). The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins (2,3). Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol (3).

Recommended Applications

WB, IHC, ICC, FC, IP

Applications and Recommended Dilution Factors

WB: 1:1,000
IHC: 1:500
ICC: 1:500
FC: 1:40
IP: 1:40

Species Reactivity

Human, Mouse, Rat

Products Data

A. Western blot analysis on Hela cell lysate using anti-14-3-3 b/a (C-term) RabMAb (cat. #1062-1), dilution 1:1,000.

B. Immunohistochemical analysis of paraffin-embedded breast tissue anti-14-3-3 b/a (C-term) RabMAb (cat. #1062-1).

  

Specificity

A synthetic peptide corresponding to C-terminal residues of human 14-3-3 b/a was used as immunogen.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Alternative Names

YWHAB, 14-3-3 protein beta/alpha, N-terminally processed, Protein 1054

Description References

1. Fu, H., et al. 14-3-3 proteins: structure, function, and regulation. Ann. Rev. Pharmacol. Toxicol. 40: 617
2. Muslin, A.J. et al. Interaction of 14-3-3 with Signaling Proteins Is Mediated by the Recognition of Phosphoserine. Cell 84: 889
3. Zha, J. et al. Serine Phosphorylation of Death Agonist BAD in Response to Survival Factor Results in Binding to 14-3-3 Not BCL-XL. Cell 87: 619