文件名：/images/down/2010031609180833.pdf
发布时间：2017-9-20 9:19:22
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Test Principle
The test is an indirect competitive ELISA that allows the congener-independent detection of
microcystins and nodularins. It is based on the recognition of microcystins, nodularins and
their congeners by specific antibodies. Microcystins, nodularins and their congeners when
present in a sample and a microcystins-protein analogue immobilized on the plate compete
for the binding sites of antibodies in solution. After a washing step, a second antibody-HRP
label is added. After a washing step and addition of the substrate solution, a color signal is
generated. The intensity of the blue color is inversely proportional to the concentration of the
microcystins present in the sample. The color reaction is stopped after a specified time and
the color is evaluated using an ELISA reader.
