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Lightning-Link HRP conjugation kit/辣根过氧化物酶

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发布时间:2014-12-23 9:18:50

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Lightning-Link Horseradish Peroxidase Labeling Kit

Lightning-Link
Lightning-Link is the easiest and quickest method available for making antibody (protein) conjugates, requiring just 30 seconds hands-on time. The antibody is covalently bonded to the label in a directional and controlled process at near-neutral pH.

Horseradish Peroxidase
Enzyme conjugates (such as antibody-HRP) give a highly sensitive and specific immuno-reagent. Horseradish peroxidase is frequently the label of choice for ELISA, immunoblotting and immunocytochemistry, due to its small size (40kDa) and high turnover rate, producing a measurable result over a short time period.

Lightning-Link HRP pricing

  • 30 seconds hands-on time
  • No separation steps
  • No losses

Click to see a titration of a typical monoclonal antibody prepared using Lightning-Link and a standard method


Feature
Kit A
Kit B
Kit C
Kit D
Kit E
Lightning-Link
Hands-on time
1 h
2 h
30 min
2 h
2 h
30 seconds
# dialysis/desalting steps
2
2
1
2
2
0
# kit components
5
7
4
5
2
3
# reagents not supplied
0
0
0
1
3
0
Freely scalable
No
No
No
No
No
Yes
Conjugation pH range
9.4 or 7.2
9.6
9.3-9.5
9.5
9.5
6.8-7.8